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KMID : 0380620080400050593
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Korean Journal of Food Science and Technology
2008 Volume.40 No. 5 p.593 ~ p.598
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Isolation and Elucidation of Specific RNAs by Treatment of Rhus verniciflua Stokes Extract to U937 Cell
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Jung Mi-Young
Oh Se-Wook
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Abstract
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Differential display RT-PCR was used for screening the differentially expressed specific genes by Rhus verniciflua extract treatment to U937 cell, human leukemic monocyte. As a result, 19 clones differentially expressed were detected. Among the detected clones, one clone was confirmed to be over-expressed by R. verniciflua extract treatment in Northern blot analysis. Nucleotide sequence of the clone showed 100% homology with H2A histone family member Z gene. Therefore, it is concluded that the treatment of R. verniciflua extract to U937 cell specifically induces the expression of H2A.Z gene but its role should be elucidated by future works.
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KEYWORD
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specific RNA, differential display, H2A.Z gene, Rhus verniciflua, extract
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